D-キシロースレダクターゼ

D-キシロースレダクターゼ
識別子
EC番号	1.1.1.307
データベース
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB構造	RCSB PDB PDBj PDBe PDBsum
検索 PMC articles PubMed articles NCBI proteins

D-キシロースレダクターゼ(D-xylose reductase)は、次の化学反応を触媒する酵素である。

キシリトール + NAD(P)⁺ $\rightleftharpoons$ D-キシロース + NAD(P)H + H⁺

この酵素の基質はキシリトールとNAD⁺(NADP⁺)で、生成物はD-キシロース、NAD(P)HとH⁺である。

この酵素は酸化還元酵素に属し、NAD⁺またはNADP⁺を受容体として供与体であるCH-OH基に特異的に作用する。組織名はxylitol:NAD(P)⁺ oxidoreductaseで、別名にXylR、XyrA、msXR、dsXR、monospecific xylose reductase、dual specific xylose reductase、NAD(P)H-dependent xylose reductase、xylose reductaseがある。

参考文献

• Neuhauser, W., Haltrich, D., Kulbe, K.D. and Nidetzky, B. (1997). “NAD(P)H-dependent aldose reductase from the xylose-assimilating yeast Candida tenuis. Isolation, characterization and biochemical properties of the enzyme.”. Biochem. J. 326: 683-692. PMID 9307017. 
• Nidetzky, B., Bruggler, K., Kratzer, R. and Mayr, P. (2003). “Multiple forms of xylose reductase in Candida intermedia: comparison of their functional properties using quantitative structure-activity relationships, steady-state kinetic analysis, and pH studies.”. J. Agric. Food Chem. 51: 7930-7935. PMID 14690376. 
• Iablochkova, E.N., Bolotnikova, O.I., Mikhailova, N.P., Nemova, N.N. and Ginak, A.I. (2003). “The activity of xylose reductase and xylitol dehydrogenase in yeasts.”. Mikrobiologiia 72: 466-469. PMID 14526534. (in Russian)
• Chen, L.C., Huang, S.C., Chuankhayan, P., Chen, C.D., Huang, Y.C., Jeyakanthan, J., Pang, H.F., Men, L.C., Chen, Y.C., Wang, Y.K., Liu, M.Y., Wu, T.K. and Chen, C.J. (2009). “Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from Candida tropicalis.”. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 65: 419-421. PMID 19342796. 
• Verduyn, C., Van Kleef, R., Frank, J., Schreuder, H., Van Dijken, J.P. and Scheffers, W.A. (1985). “Properties of the NAD(P)H-dependent xylose reductase from the xylose-fermenting yeast Pichia stipitis.”. Biochem. J. 226: 669-677. PMID 3921014. 
• Fernandes, S., Tuohy, M.G. and Murray, P.G. (2009). “Xylose reductase from the thermophilic fungus Talaromyces emersonii: cloning and heterologous expression of the native gene (Texr) and a double mutant (TexrK271R + N273D) with altered coenzyme specificity.”. J Biosci 34: 881-890. PMID 20093741. 
• Lee, J.K., Koo, B.S. and Kim, S.Y. (2003). “Cloning and characterization of the xyl1 gene, encoding an NADH-preferring xylose reductase from Candida parapsilosis, and its functional expression in Candida tropicalis.”. Appl. Environ. Microbiol. 69: 6179-6188. PMID 14532079. 
• Woodyer, R., Simurdiak, M., van der Donk, W.A. and Zhao, H. (2005). “Heterologous expression, purification, and characterization of a highly active xylose reductase from Neurospora crassa.”. Appl. Environ. Microbiol. 71: 1642-1647. PMID 15746370.